999国产精品999久久久久久,香港经典a毛片免费观看,国产av在线私拍,97se亚洲国产综合在线

當(dāng)前位置:首頁  >  技術(shù)文章  >  DDIT3對Luminal A型乳腺癌的影響

DDIT3對Luminal A型乳腺癌的影響

更新時(shí)間:2024-12-28  |  點(diǎn)擊率:100

20237月,黑龍江省科學(xué)院先進(jìn)技術(shù)研究所;黑龍江省科學(xué)院先進(jìn)技術(shù)研究所(Institute of Advanced Technology, Heilongjiang Academy of SciencesInstitute of Advanced Technology, Heilongjiang Academy of Sciences) Guoqing Huang老師研究團(tuán)隊(duì)在《Research Square》上發(fā)表論文:

The effect of DDIT3 on luminal A type breast cancer"

 

DDIT3Luminal A型乳腺癌的影響"

 

Abstract

Purpose: To analyze the phenotypic changes of breast cancer (BC) cell before and after DDIT3 knockdown/overexpression, and preliminarily explore the regulatory mechanism. Also, to analyze the relationship between DDIT3 and prognosis by combining with bioinformatics methods, which provide a basis for further research on DDIT3 targeted treatment of BC.

Methods: Loss- and gain-of-function studies, DDIT3 in MCF-7 (luminal A), and RNA-seq analysis were employed to investigate the functional impact of DDIT3 on BC cell proliferation and drug resistance. The relationship between DDIT3 and the prognosis of BC patients was systematically assessed using the tissue microarray technique along with qRT-PCR and publicly available data.

Results: Survival analysis showed that patients with lower DDIT3 expression in luminal A type BC or BC patient which were undergoing endocrine therapy had a poorer prognosis, and DDIT3 expression was associated with overall survival (OS) significant. Following the knockdown of DDIT3 in MCF-7 cells, the proliferation rate was significantly increased, and drug resistance ability was just reversed. On the contrary, overexpression of DDIT3 had a relative inhibitory effect on target cell proliferation. Notably, the inhibition of DDIT3 expression upregulated TP63 and downregulated PDGFR, with the results being exactly opposite after the overexpression of DDIT3.

Conclusion: These results have revealed that DDIT3 plays a critical role in luminal A type BC cell proliferation and TAM resistance, and it holds potential prognostic value in BC. Overall, DDIT3 may exert its functions in luminal A type BC by modulating the expression of TP63 and PDGFR.


摘要:

目的:分析乳腺癌(BC)細(xì)胞DDIT3敲低/過表達(dá)前后的表型變化,并初步探討其調(diào)控機(jī)制。結(jié)合生物信息學(xué)方法分析DDIT3與預(yù)后的關(guān)系,為進(jìn)一步研究DDIT3靶向治療BC提供依據(jù)。

方法:通過功能缺失和功能獲得研究、MCF-7 (luminal A)中的DDIT3RNA-seq分析來研究DDIT3BC細(xì)胞增殖和耐藥的功能影響。利用組織微陣列技術(shù)、qRT-PCR和公開數(shù)據(jù)系統(tǒng)評估DDIT3BC患者預(yù)后的關(guān)系。

結(jié)果:生存分析顯示,在Luminal A BC或接受內(nèi)分泌治療的BC患者中,DDIT3表達(dá)較低的患者預(yù)后較差,且DDIT3表達(dá)與總生存(OS)顯著相關(guān)。MCF-7細(xì)胞中敲低DDIT3后,增殖速率明顯提高,耐藥能力剛好逆轉(zhuǎn)。相反,過表達(dá)DDIT3對靶細(xì)胞增殖有相對抑制作用。值得注意的是,抑制DDIT3表達(dá)可上調(diào)TP63,下調(diào)PDGFR,而過表達(dá)DDIT3后的結(jié)果正好相反。

結(jié)論:這些結(jié)果揭示了DDIT3Luminal A BC細(xì)胞增殖和TAM耐藥中起關(guān)鍵作用,并具有潛在的預(yù)后價(jià)值。綜上所述,dddit3可能通過調(diào)節(jié)TP63PDGFR的表達(dá)而在luminal ABC中發(fā)揮作用。

 

該論文中,HEK293T和人乳腺癌(BC)細(xì)胞系MCF-7的體外培養(yǎng)是使用Ausbian特級胎牛血清完成的。


午夜精品久久久久久久无码 | 久久夜色精品国产噜噜av| 精品久久久久久久无码人妻热| 欧美艳星nikki激情办公室| 男男大尺度无码a片在线观看 | 熟妇人妻无乱码中文字幕| 99精品国产99久久久久久97| 精品国产乱码久久久久久1区2区 | 色一情一区二| 国内精品久久人妻无码hd| 成人午夜性a级毛片免费| 老司机午夜福利视频| 久久无码中文字幕东京热| 国产精品点击进入在线影院高清| 少妇高潮喷水久久久久久久久久| 啦啦啦中文免费观看在线| 久久久久久综合网天天| 东北妇女精品bbwbbw| 国产在线精品一区二区三区不卡 | 99久久精品费精品国产一区二| 日本免费无码一区二区到五区| 欧美变态口味重另类在线视频| 少妇的丰满3中文字幕 | 欧美后进式猛烈xx00免费视频| 亚洲国产精品日韩AV专区| 人妻激情偷乱视频一区二区三区| 亚洲人成无码网站久久99热国产| 欧洲熟妇色xxxx欧美老妇多毛| 亚洲精品无码久久久久久| 国产亚洲精品久久久一区| 久久久久久精品免费免费999| 粉嫩极品国产在线观看| 亚洲精品欧美综合一区二区 | 国产乱人伦精品一区二区| 色欲老女人人妻综合网| 久久综合亚洲欧美成人| 久久www免费人成一看片| 国产色无码专区在线观看| 国产精品人妻一区二区三区四区| 一本久道中文无码字幕av| 亚洲成a人片在线观看www|